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What are the challenges associated with using circulating free DNA as a liquid biopsy tool? Challenges Associated with Using Circulating Free DNA as a Liquid Biopsy Tool One of the Circulating Free DNA main challenges associated with using circulating free DNA (cfDNA) as a liquid biopsy tool is the potential for false positives or false negatives. The detection and analysis of cfDNA can be complex, and errors in interpretation can occur. This can lead to incorrect diagnoses or treatment decisions. Another chal... more